ALOX5 anticorps (pSer523)

N° du produit ABIN372586

Cet anticorps Lapin Polyclonal détecte spécifiquement ALOX5 dans WB. Il présente une réactivité envers Humain, Rat et Primate.

Aperçu rapide pour ALOX5 anticorps (pSer523) (ABIN372586)

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivité: Humain, Rat, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ALOX5 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-ALOX5 anticorps

Épitope: pSer523

Specificité: Recognizes the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Réactivité croisée (Details): Species reactivity (expected):non-Human Primates.
Species reactivity (tested):Human and Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser523 of human 5-Lipoxygenase (5-LO).

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur ALOX5

Antigène: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Autre désignation: ALOX5 / LOG5

Sujet: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

ID gène: 240

NCBI Accession: NP_000689

UniProt: P09917