GAP43 anticorps (pSer41)

N° du produit ABIN372649

Détail du produit anti-GAP43 anticorps

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Épitope: pSer41

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Poisson zèbre (Danio rerio), Chien, Xenopus laevis, Pinson, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAP43 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Specific for the ~50k Gap-43 protein phosphorylated at Ser41 in Western Blots of Rat brain lysate. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with lambda-Ptase.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine, Chicken, Finch, Human, Mouse, non-Human Primates, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemitsry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur GAP43

Antigène: GAP43 (Growth Associated Protein 43 (GAP43))

Autre désignation: GAP43 (GAP43 Produits)

Synonymes: anticorps B-50, anticorps PP46, anticorps Basp2, anticorps GAP-43, anticorps b-50, anticorps gap-43, anticorps gap43b, anticorps pp46, anticorps cb310, anticorps gap, anticorps gap43, anticorps gap43a, anticorps growth associated protein 43, anticorps growth associated protein 43 L homeolog, anticorps growth associated protein 43 S homeolog, anticorps GAP43, anticorps Gap43, anticorps gap43.L, anticorps gap43, anticorps gap43.S

Sujet: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).Synonyms: Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46

ID gène: 29423

NCBI Accession: NP_058891

UniProt: P07936