MAP2
Reactivité: Rat
WB, IHC, ICC, IP
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blot: 1/20,000. Immunohistochemistry. Immunofluorescence: 1/2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at-20 °C.
Klüssendorf, Song, Schau, Morellini, Dityatev, Koliwer, Kreienkamp: "The Golgi-Associated PDZ Domain Protein Gopc/PIST Is Required for Synaptic Targeting of mGluR5." dans: Molecular neurobiology, Vol. 58, Issue 11, pp. 5618-5634, (2022) (PubMed).
Jacquemet, Paatero, Carisey, Padzik, Orange, Hamidi, Ivaska: "FiloQuant reveals increased filopodia density during breast cancer progression." dans: The Journal of cell biology, Vol. 216, Issue 10, pp. 3387-3403, (2017) (PubMed).
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker