MAP2 anticorps

N° du produit ABIN372661

Cet anticorps Poulet Polyclonal détecte spécifiquement MAP2 dans WB, IF et IHC (fro). Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Boeuf (Vache). Il a été cité dans 2+ publications.

Aperçu rapide pour MAP2 anticorps (ABIN372661)

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-MAP2 anticorps

Specificité: This antibody is specific for the ~280k MAP2 protein.

Réactivité croisée (Details): Species reactivity (tested):Bovine, Human, Mouse and Rat.

Purification: Ig Fraction

Immunogène: Bovine MAP2 isolated from brain by the GTP microtubule cycling method.

Isotype: Ig Fraction

Information d'application

Indications d'application: Western blot: 1/20,000. Immunohistochemistry. Immunofluorescence: 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-MAP2 (ABIN372661)

Klüssendorf, Song, Schau, Morellini, Dityatev, Koliwer, Kreienkamp: "The Golgi-Associated PDZ Domain Protein Gopc/PIST Is Required for Synaptic Targeting of mGluR5." dans: Molecular neurobiology, Vol. 58, Issue 11, pp. 5618-5634, (2022) (PubMed).

Jacquemet, Paatero, Carisey, Padzik, Orange, Hamidi, Ivaska: "FiloQuant reveals increased filopodia density during breast cancer progression." dans: The Journal of cell biology, Vol. 216, Issue 10, pp. 3387-3403, (2017) (PubMed).

Détails sur MAP2

Antigène: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Autre désignation: MAP-2

Sujet: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau. MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

ID gène: 4133

NCBI Accession: NP_002365

UniProt: P11137