GRIN1/NMDAR1 anticorps (Splice Variant C2)

N° du produit ABIN372676

Cet anticorps Lapin Polyclonal détecte spécifiquement GRIN1/NMDAR1 dans WB et IHC (fro). Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour GRIN1/NMDAR1 anticorps (Splice Variant C2) (ABIN372676)

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRIN1/NMDAR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GRIN1/NMDAR1 anticorps

Épitope: Splice Variant C2

Specificité: This antibody recognizes the ~120k NR1 subunit of the NMDA Receptor containing the C2 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C2 insert.

Réactivité croisée (Details): Species reactivity (expected):Human.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography.

Immunogène: Peptide from the NR1 subunit, C2 splice variant insert of Rat NMDA Receptor.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Références pour anticorps anti-GRIN1/NMDAR1 (ABIN372676)

Brady, Diaz, Iuso, Everett, Valenzuela, Caldwell: "Moderate prenatal alcohol exposure reduces plasticity and alters NMDA receptor subunit composition in the dentate gyrus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 3, pp. 1062-7, (2013) (PubMed).

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." dans: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, (2001) (PubMed).

Détails sur GRIN1/NMDAR1

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Autre désignation: NMDA Receptor 1

Sujet: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). There are a number of different splice variants of the NR1 subunit (Foldes et al., 1994, Zukin and Bennett, 1995). Differential splicing of three exons in the NR1 subunit generates up to eight NR1 subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1

ID gène: 24408

NCBI Accession: NP_058706

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity