GRIN1/NMDAR1 anticorps (Splice Variant N1)

N° du produit ABIN372678

Cet anticorps anti-GRIN1/NMDAR1 est un anticorps Lapin Polyclonal détectant GRIN1/NMDAR1 dans WB et IHC (fro). Adapté pour Humain, Rat, Souris, Chien, Xenopus laevis, Poulet et Non-Human Primate.

Aperçu rapide pour GRIN1/NMDAR1 anticorps (Splice Variant N1) (ABIN372678)

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivité: Humain, Rat, Souris, Chien, Xenopus laevis, Poulet, Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GRIN1/NMDAR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GRIN1/NMDAR1 anticorps

Épitope: Splice Variant N1

Specificité: This antibody recognizes the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA Receptor. It Does not recognize NR1 subunits of the NMDA Receptor that do not contain the N1 insert.

Réactivité croisée (Details): Species reactivity (expected):Canine, Chicken, Human, Xenopus and non-Human Primates.
Species reactivity (tested):Mouse and Rat.

Purification: Affinity Chromatography.

Immunogène: Peptide from the NR1 subunit, N1 splice variant insert of Rat NMDA Receptor.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1000. Immunohistochemistry on Frozen Sections: 1/1000-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur GRIN1/NMDAR1

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Autre désignation: NMDA Receptor 1

Sujet: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.Synonyms: GRIN1, Glutamate [NMDA] receptor subunit zeta-1, NMDAR1

ID gène: 24408

NCBI Accession: NP_058706

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity