RPH3A anticorps (pSer234)

N° du produit ABIN372710

L’anticorps Lapin Polyclonal anti-RPH3A a été validé pour WB et IHC (fro). Il convient pour détecter RPH3A dans des échantillons de Souris et Rat.

Aperçu rapide pour RPH3A anticorps (pSer234) (ABIN372710)

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPH3A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-RPH3A anticorps

Épitope: pSer234

Specificité: This antibody recognizes the ~82k Rabphilin protein phosphorylated at Ser234. The immunolabeling of the Rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of Rabphilin 3A.

Isotype: IgG

Information d'application

Indications d'application: Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur RPH3A

Antigène: RPH3A (Rabphilin 3A (RPH3A))

Autre désignation: Rabphilin-3A / RPH3A

Sujet: Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994, Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001).Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A

ID gène: 171039

NCBI Accession: NP_598202

UniProt: P47709

Pathways: Synaptic Vesicle Exocytosis