SYT1 anticorps (pThr202)

N° du produit ABIN372724

Cet anticorps Lapin Polyclonal détecte spécifiquement SYT1 dans WB, IHC (fro) et DB. Il présente une réactivité envers Humain, Rat, Souris, Boeuf (Vache), Poulet, Chien, Poisson zèbre (Danio rerio) et Non-Human Primate.

Aperçu rapide pour SYT1 anticorps (pThr202) (ABIN372724)

Antigène: SYT1 (Synaptotagmin I (SYT1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Poulet, Chien, Poisson zèbre (Danio rerio), Non-Human Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SYT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Détail du produit anti-SYT1 anticorps

Épitope: pThr202

Specificité: This antibody recognizes the ~60k-62k synaptotagmin protein phosphorylated at Thr202 in Western Blots of Rat brain lysate. The immunolabeling is completely eliminated by lambda-phosphatase treatment.

Réactivité croisée (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primate and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of Synaptotagmin.

Isotype: IgG

Information d'application

Indications d'application: Dot blot: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur SYT1

Antigène: SYT1 (Synaptotagmin I (SYT1))

Autre désignation: Synaptotagmin-1

Sujet: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001, Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999, Lee et al., 2004).Synonyms: SYT1, SYT2, SYT3, SYT4, SYT5

ID gène: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport