Tryptophan Hydroxylase 1 anticorps

N° du produit ABIN372730

Détail du produit anti-Tryptophan Hydroxylase 1 anticorps

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Reactivité: Humain, Rat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Specific for the ~55k Tryptophan Hydroxylase protein. The antibody has been directly tested for reactivity in Western blots with Human and Rat tissue. Based upon the relatively high degree of homology of Tryptophan Hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in Rabbit tissues.

Réactivité croisée (Details): Species reactivity (tested):Human and Rat.

Purification: Affinity Chromatography.

Immunogène: Recombinant rabbit Tryptophan Hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1/1,000. Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur Tryptophan Hydroxylase 1

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Autre désignation: Tryptophan 5-Hydroxylase 1 (TPH1) (TPH1 Produits)

Synonymes: anticorps TPRH, anticorps TRPH, anticorps tph, anticorps tph1, anticorps tphD1, anticorps tph2, anticorps tph1l, anticorps tphD2, anticorps Tph, anticorps TPH, anticorps SERGEF, anticorps LOC100229598, anticorps tryptophan hydroxylase 1, anticorps tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a, anticorps TryPtophan Hydroxylase, anticorps tryptophan hydroxylase 1b, anticorps tryptophan hydroxylase 1 L homeolog, anticorps tryptophan 5-hydroxylase 1, anticorps TPH1, anticorps tph1a, anticorps tph-1, anticorps tph1b, anticorps Tph1, anticorps tph1.L, anticorps LOAG_02149, anticorps Tsp_05203

Sujet: Tryptophan Hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).Synonyms: TPRH, TRPH, Tryptophan 5-monooxygenase 1

ID gène: 7166

NCBI Accession: NP_004170

UniProt: P17752

Pathways: Feeding Behaviour