Tryptophan Hydroxylase 2 anticorps (pSer19)

N° du produit ABIN372731

Détail du produit anti-Tryptophan Hydroxylase 2 anticorps

Antigène: Tryptophan Hydroxylase 2 (TPH2)

Épitope: pSer19

Reactivité: Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 2 est non-conjugé

Application: Western Blotting (WB)

Specificité: Specific for the ~55k Tryptophan Hydroxylase protein phosphorylated at Ser19.

Réactivité croisée (Details): Species reactivity (expected):Bovine, Mouse and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography via Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogène: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser19 of rat Tryptophan Hydroxylase 2 (TPH2).

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C.

Détails sur Tryptophan Hydroxylase 2

Antigène: Tryptophan Hydroxylase 2 (TPH2)

Autre désignation: Tryptophan 5-Hydroxylase 2 (TPH2) (TPH2 Produits)

Synonymes: anticorps ADHD7, anticorps NTPH, anticorps Ntph, anticorps tphR, anticorps wu:fq15a04, anticorps AU043594, anticorps tryptophan hydroxylase 2, anticorps tryptophan hydroxylase 2 (tryptophan 5-monooxygenase), anticorps TPH2, anticorps Tph2, anticorps tph2

Sujet: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000, Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).Synonyms: NTPH, Neuronal tryptophan hydroxylase, Tryptophan 5-monooxygenase 2

ID gène: 317675

NCBI Accession: NP_776211

UniProt: Q8CGU9