HPSE anticorps

N° du produit ABIN372789

Cet anticorps anti-HPSE Polyclonal Lapin (ABIN372789) détecte spécifiquement HPSE dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour HPSE anticorps (ABIN372789)

Antigène: HPSE (Heparanase (HPSE))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HPSE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HPSE anticorps

Specificité: Recognizes the 65 kDa precursor as well as the 55 kDa and 5 kDa subunits of Human or Mouse Heparanase.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogène: Human 50kD-8kD Heparanase Heterodimer.

Isotype: IgG

Information d'application

Indications d'application: Western Blot: 1/500. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This HPSE antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody Rabbit IgG, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.001 % Thimerosal as preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur HPSE

Antigène: HPSE (Heparanase (HPSE))

Autre désignation: Heparanase / HPSE

Sujet: Heparanase is an endo-beta-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes. Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis. Heparanase is a heterodimer comprised of a 50kD subunit harboring the active site and a 8kD subunit. It is produced as a latent 65kD precursor and proteolytically processed to its active form. Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival.Synonyms: Endo-Glucoronidase, HEP, HPA, HPA1, HPR1, HPSE1, HSE1, Heparanase-1

ID gène: 10855

NCBI Accession: NP_001092010

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process