PC anticorps (Internal Region)

N° du produit ABIN372799

Cet anticorps Chèvre Polyclonal détecte spécifiquement PC dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Porc, Boeuf (Vache) et Chien.

Aperçu rapide pour PC anticorps (Internal Region) (ABIN372799)

Antigène: PC (Pyruvate Carboxylase (PC))

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PC anticorps

Épitope: Internal Region

Specificité: Recognizes Pyruvate Carboxylase (PC).

Réactivité croisée (Details): Species reactivity (expected):Bovine, Canine (Dog), Mouse, Porcine and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide (C-KFKEVKKAYVEANQ) from an Internal region of Human PC.

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/16000. Western blot: 0.03-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PC

Antigène: PC (Pyruvate Carboxylase (PC))

Autre désignation: Pyruvic Carboxylase (PC)

Sujet: Pyruvate Carboxylase (PC, PCB) requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. PCB is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.Synonyms: PCB, Pyruvate carboxylase mitochondrial

ID gène: 5091

NCBI Accession: NP_000911

UniProt: P11498