MELK anticorps (C-Term)

N° du produit ABIN374073

Cet anticorps anti-MELK est un anticorps Chèvre Polyclonal détectant MELK dans WB et EIA. Adapté pour Humain, Souris, Boeuf (Vache) et Chien.

Aperçu rapide pour MELK anticorps (C-Term) (ABIN374073)

Antigène: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Reactivité: Humain, Souris, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MELK est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-MELK anticorps

Épitope: C-Term

Séquence: KRLVEDILSS CKV

Specificité: This antibody detects MELK at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Canine (Dog), Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Syntehtic Peptide from the C Terminus of the protein sequence according to NP_055606.1. Genename: MELK

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3´μg/mL. Experiments gave bands at approx 75 kDa and 150 kDa inhuman testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The 150 kDa band is explained by glycosylation of this member of the AMPK family. Thisprotein has a calculated MW of 74.6 kDa according to NP_055606.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containng 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MELK

Antigène: MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))

Autre désignation: MELK

Sujet: MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine. MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK

ID gène: 9833

NCBI Accession: NP_055606

UniProt: Q14680