B-Cell Linker anticorps (C-Term)

N° du produit ABIN374092

Cet anticorps Chèvre Polyclonal détecte spécifiquement B-Cell Linker dans WB et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour B-Cell Linker anticorps (C-Term) (ABIN374092)

Antigène: B-Cell Linker (BLNK)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp B-Cell Linker est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-B-Cell Linker anticorps

Épitope: C-Term

Séquence: C-KDSTRLKYAV KVS

Specificité: Detects B-cell linker protein/BLNK at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1 Genename: BLNK

Information d'application

Indications d'application: Peptide ELISA: BLNK antibody Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 70 kDa band observed in lysates of cell line Daudi(calculated MW of 50.0 kDa according to 037446.1). The observed molecular weightcorresponds to how SLP-65 is expected to run. However, when transiently expressed inHEK293, a 50 kDa band is observed.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: B-Cell Linker Protein / BLNK

Sujet: BLNK (B cell linker protein) is an adaptor molecule that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). This activation leads to an increase in the tyrosine phosphorylation of a number of proteins due to activation of nonreceptor protein tyrosine kinases (PTK) including Syk, which is responsible for the tyrosine phosphorylation of BLNK. BLNK is cytosolic protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human BLNK). Mapping of the tyrosine residues in BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines that bind the Tec family kinase Btk, the Vav nucleotide exchange factor and the adaptor Nck.Synonyms: B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, BASH, Cytoplasmic adapter protein, SLP-65, SLP65, Src homology 2 domain-containing leukocyte protein of 65 kDa

ID gène: 29760

NCBI Accession: NP_037446

UniProt: Q8WV28

Pathways: BCR Signaling