PLK4 anticorps (N-Term)
Aperçu rapide pour PLK4 anticorps (N-Term) (ABIN374175)
Antigène
Voir toutes PLK4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- N-Term
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Séquence
- ATCIGEKIED FKVGN
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Specificité
- This antibody detects SAK / STK18 at N-term.
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Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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Immunogène
- Peptide from N Terminus of the protein sequence according to NP_055079.3 Genename: PLK4
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Indications d'application
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 95 kDa band observed in Human Placenta lysates(calculated MW of 105 kDa according to NP_055079.2). An additional faint band of 55 kDawas consistently observed, however this band was not blocked by the immunizing peptideand it is therefore a non-specific signal. We call for caution when used for other assaysthan Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- PLK4 (Polo-Like Kinase 4 (PLK4))
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Autre désignation
- PLK4 / SAK / STK18
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Classe de substances
- Phage Protein
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Sujet
- Synonyms: PLK-4, Polo-like kinase 4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase PLK4, Serine/threonine-protein kinase Sak
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ID gène
- 10733
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NCBI Accession
- NP_055079
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UniProt
- O00444
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Pathways
- M Phase
Antigène
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