PEBP1 anticorps (C-Term)

N° du produit ABIN374198

L’anticorps anti-PEBP1 Polyclonal Chèvre est utilisé pour la détection de PEBP1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour PEBP1 anticorps (C-Term) (ABIN374198)

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PEBP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PEBP1 anticorps

Épitope: C-Term

Séquence: C-DYVPKLYEQL SGK

Specificité: This antibody detects PBP / RKIP at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from C Terminus of the protein sequence according to NP_002558.1 Genename: PEBP1

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 22 kDa band observed in lysates of Human Kidneyfibroblast HEK293, Human lung carcinoma A549 and Human Hepatoblastoma HepG2 celllines (calculated MW of 21.2 kDa according to NP_002558.1). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PEBP1

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Autre désignation: PEBP1 / RKIP

Sujet: PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.Synonyms: HCNPpp, PBP, PEBP, PEBP-1, Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, Raf kinase inhibitor protein

ID gène: 5037

NCBI Accession: NP_002558

UniProt: P30086

Pathways: Feeding Behaviour