NDC80 anticorps (C-Term)
Aperçu rapide pour NDC80 anticorps (C-Term) (ABIN374264)
Antigène
Voir toutes NDC80 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Séquence
- C-YEKKATLIKS SEE
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Specificité
- This antibody detects NDC80 at C-term.
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Réactivité croisée (Details)
- Species reactivity (tested):Human
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Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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Immunogène
- Peptide from C Terminus of the protein sequence according to NP_006092.1 Genename: NDC80
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Indications d'application
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 75-80 kDa band observed in Hela lysate (predicted MWof 70 kDa according to NP_006092).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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Autre désignation
- NDC80
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Sujet
- HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.Synonyms: HEC, HEC1, KNTC2
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ID gène
- 10403
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NCBI Accession
- NP_006092
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UniProt
- O14777
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Pathways
- Maintenance of Protein Location
Antigène
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