PDIA3 anticorps (C-Term)
Aperçu rapide pour PDIA3 anticorps (C-Term) (ABIN374286)
Antigène
Voir toutes PDIA3 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Séquence
- C-EEKPKKKKKA QEDL
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Specificité
- This antibody detects PDIA3 at C-term.
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Réactivité croisée (Details)
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Species reactivity (expected):Rat and Cow.
Species reactivity (tested):Human, Mouse. -
Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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Immunogène
- Peptidefrom C Terminus of the protein sequence according to NP_005304.3 Genename: PDIA3
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Indications d'application
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Peptide ELISA: Limit Dilution 1/8000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in human hepatoblastoma HepG2lysates (calculated MW of 56.5 kDa according to NP_005304.3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))
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Autre désignation
- PDIA3
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Sujet
- ERp57, also known as GRP58, PDIA3, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.Synonyms: Disulfide isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, Protein disulfide-isomerase A3
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ID gène
- 2923
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NCBI Accession
- NP_005304
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UniProt
- P30101
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Pathways
- Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
Antigène
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