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PDIA3 anticorps (C-Term)

Cet anticorps anti-PDIA3 est un anticorps Chèvre Polyclonal détectant PDIA3 dans WB et EIA. Adapté pour Humain, Souris, Rat et Boeuf (Vache).
N° du produit ABIN374286

Aperçu rapide pour PDIA3 anticorps (C-Term) (ABIN374286)

Antigène

Voir toutes PDIA3 Anticorps
PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité

  • 90
  • 36
  • 33
  • 7
  • 7
  • 6
  • 6
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat, Boeuf (Vache)

Hôte

  • 72
  • 30
  • 3
Chèvre

Clonalité

  • 67
  • 38
Polyclonal

Conjugué

  • 45
  • 8
  • 8
  • 6
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp PDIA3 est non-conjugé

Application

  • 83
  • 37
  • 33
  • 29
  • 20
  • 19
  • 13
  • 13
  • 13
  • 10
  • 5
  • 3
  • 2
  • 2
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Épitope

    • 17
    • 15
    • 11
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Séquence

    C-EEKPKKKKKA QEDL

    Specificité

    This antibody detects PDIA3 at C-term.

    Réactivité croisée (Details)

    Species reactivity (expected):Rat and Cow.
    Species reactivity (tested):Human, Mouse.

    Purification

    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

    Immunogène

    Peptidefrom C Terminus of the protein sequence according to NP_005304.3 Genename: PDIA3
  • Indications d'application

    Peptide ELISA: Limit Dilution 1/8000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in human hepatoblastoma HepG2lysates (calculated MW of 56.5 kDa according to NP_005304.3).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

    Autre désignation

    PDIA3

    Sujet

    ERp57, also known as GRP58, PDIA3, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.Synonyms: Disulfide isomerase ER-60, ER marker, ERP57, ERP60, GRP58, Protein Disulfide Isomerase, Protein disulfide-isomerase A3

    ID gène

    2923

    NCBI Accession

    NP_005304

    UniProt

    P30101

    Pathways

    Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis
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