Midline 2 anticorps (C-Term)

N° du produit ABIN374299

Cet anticorps Chèvre Polyclonal détecte spécifiquement Midline 2 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Porc.

Aperçu rapide pour Midline 2 anticorps (C-Term) (ABIN374299)

Antigène: Midline 2 (MID2)

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Midline 2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Midline 2 anticorps

Épitope: C-Term

Séquence: QESPYVSGMK TCH

Specificité: This antibody detects RNF60 / MID2 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Rat, Dog, Pig, CowSpecies reactivity (tested):Human, Mouse

Purification: Amonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_036348.2, NP_438112.2 Genename: MID2

Information d'application

Indications d'application: Peptide ELISA: Lmit dilution 1: 32000. Western bot: 1-3 μml. Approx 80 kDa band observed in mouse heart lysates (calculated MWof 81.0 kDa according to NP_036348.1 and 77.0 kDa according to NP_438112.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining in cytoplasm and brush border of some epithelial cells of proximal convolutedtubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur Midline 2

Antigène: Midline 2 (MID2)

Autre désignation: MID2 / RNF60

Sujet: Synonyms: FXY2, Midin-2, Midline defect 2, Midline-2, Probable E3 ubiquitin-protein ligase MID2, RING finger protein 60, TRIM1, Tripartite motif-containing protein 1

ID gène: 11043

NCBI Accession: NP_036348

UniProt: Q9UJV3