ALMS1 anticorps (C-Term)

N° du produit ABIN374311

Cet anticorps Chèvre Polyclonal détecte spécifiquement ALMS1 dans WB et IHC (p). Il présente une réactivité envers Humain, Rat, Boeuf (Vache), Chien et Souris.

Aperçu rapide pour ALMS1 anticorps (C-Term) (ABIN374311)

Antigène: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Reactivité: Humain, Rat, Boeuf (Vache), Chien, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ALMS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ALMS1 anticorps

Épitope: C-Term

Séquence: C-RVTNQLLGRK VPWD

Specificité: This antibody detects ALMS1 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur ALMS1

Antigène: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Autre désignation: ALMS1

Sujet: ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328

ID gène: 7840

NCBI Accession: NP_055935

UniProt: Q8TCU4

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, M Phase