IFIH1 anticorps (N-Term)

N° du produit ABIN374329

Cet anticorps Chèvre Polyclonal détecte spécifiquement IFIH1 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour IFIH1 anticorps (N-Term) (ABIN374329)

Antigène: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFIH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-IFIH1 anticorps

Épitope: N-Term

Séquence: SNGYSTDENF RYL-C

Specificité: This antibody detects Helicard at N-term.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_071451.2. Genename: IFIH1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Immunohistochemistry: 10 μg/mL. In paraffin embedded Human Pancreas, shows strongcytoplasmic staining of some cells in the islets of Langerhans. In Western blot no signal obtained yet but low background observed in Human Placentalysate at up to 1 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur IFIH1

Antigène: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Autre désignation: Helicard

Sujet: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents, treatment with either agent alone only achieves reversible differentiation. It is believed that MDA-5 may function to promote message degradation by specific RNases. It also seems to possess growth inhibition properties. The interaction of MDA5 with double stranded RNA can lead to induction of IFN required for the the innate immune response against viruses.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116

ID gène: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response