FOXA1 anticorps (C-Term)

N° du produit ABIN374334

Cet anticorps anti-FOXA1 Polyclonal Chèvre (ABIN374334) détecte spécifiquement FOXA1 dans WB, IHC (p), ChIP et EIA. L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Boeuf (Vache) et Porc.

Aperçu rapide pour FOXA1 anticorps (C-Term) (ABIN374334)

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXA1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Détail du produit anti-FOXA1 anticorps

Épitope: C-Term

Séquence: C-GVYSRPVLNT S

Specificité: This antibody detects FOXA1.

Réactivité croisée (Details): Species reactivity (expected):Rat, Pig, Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_004487.2 Genename: FOXA1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1/16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in Human lysates of cell lineHepG2 (calculated MW of 49.0 kDa according to NP_004487.2). An additional band ofunknown identity was also consistently observed at 30 kDa. This band was successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Anonymous customers used this product successfully in ChIP with MCF7.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur FOXA1

Antigène: FOXA1 (Forkhead Box A1 (FOXA1))

Autre désignation: FOXA1 / TCF3A

Sujet: FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.Synonyms: Forkhead box protein A1, HNF-3-alpha, HNF-3A, HNF3A, Hepatocyte nuclear factor 3-alpha, TCF-3A, Transcription factor 3A

Poids moléculaire: 49.1 kDa (NP_004487.2)

ID gène: 3169

NCBI Accession: NP_004487

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis