FOXC1 anticorps (C-Term)

N° du produit ABIN374336

Il existe 3+ publications pour ce produit. L’anticorps anti-FOXC1 Polyclonal Chèvre est utilisé pour la détection de FOXC1 dans des échantillons de Humain, Souris et Poisson zèbre (Danio rerio). Il a été validé pour WB, IHC (p), ChIP et EIA.

Aperçu rapide pour FOXC1 anticorps (C-Term) (ABIN374336)

Antigène: FOXC1 (Forkhead Box C1 (FOXC1))

Reactivité: Humain, Souris, Poisson zèbre (Danio rerio)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FOXC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Détail du produit anti-FOXC1 anticorps

Épitope: C-Term

Séquence: RTSGAFVYDC SKF

Specificité: This antibody detects FOXC1 / FKHL7 / FREAC3 at C-term.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Zebrafish.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_001444.2. Genename: FOXC1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 55 kDa band observed in Human Bone Marrow lysates(calculated MW of 56.5 kDa according to NP_001444.1). Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Kidney shows nuclearstaining of some cells in the glomeruli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Références pour anticorps anti-FOXC1 (ABIN374336)

Doucette, Footz, Walter: "FOXC1 Regulates Expression of Prostaglandin Receptors Leading to an Attenuated Response to Latanoprost." dans: Investigative ophthalmology & visual science, Vol. 59, Issue 6, pp. 2548-2554, (2019) (PubMed).

Fatima, Wang, Uchida, Norden, Liu, Culver, Dietz, Culver, Millay, Mukouyama, Kume: "Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation." dans: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2437-51, (2017) (PubMed).

Ito, Goping, Berry, Walter: "Dysfunction of the stress-responsive FOXC1 transcription factor contributes to the earlier-onset glaucoma observed in Axenfeld-Rieger syndrome patients." dans: Cell death & disease, Vol. 5, pp. e1069, (2014) (PubMed).

Détails sur FOXC1

Antigène: FOXC1 (Forkhead Box C1 (FOXC1))

Autre désignation: FOXC1 / FKHL7 / FREAC3

Sujet: FOXC1 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined, however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.Synonyms: FREAC-3, Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3

Poids moléculaire: 56.8kDa (NP_001444.1).

ID gène: 2296

NCBI Accession: NP_001444

UniProt: Q12948

Pathways: Chromatin Binding, Glycosaminoglycan Metabolic Process