Anillin anticorps (C-Term)
Aperçu rapide pour Anillin anticorps (C-Term) (ABIN374360)
Antigène
Voir toutes Anillin (ANLN) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- C-Term
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Séquence
- WQPDACYKPI GKP
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Specificité
- This antibody detects Anillin / ANLN at C-term.
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Cow.
Species reactivity (tested):Human. -
Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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Immunogène
- Peptide from C Terminus of the protein sequence according to NP_061155.2. Genename: ANLN
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Indications d'application
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Peptide ELISA: Limit dilution 1: 64000. Western blot: Experiments gave an approx 75 kDa band in Mouse Brain lysates after 0.3 μg/mL antibody staining. It was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 3-10 μg/mL. In paraffin embedded Human Kidney shows staining ofnuclei in some cells of renal tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))
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Autre désignation
- Anillin / ANLN
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Sujet
- Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.Synonyms: Actin-binding protein anillin
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ID gène
- 54443
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NCBI Accession
- NP_061155
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UniProt
- Q9NQW6
Antigène
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