KPNA1 anticorps (N-Term)

N° du produit ABIN374391

Détail du produit anti-KPNA1 anticorps

Antigène: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Épitope: N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNA1 est non-conjugé

Application: Western Blotting (WB)

Séquence: TTPGKENFRL KSY-C

Specificité: This antibody detects Importin subunit alpha-1 at N-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur KPNA1

Antigène: KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

Autre désignation: KPNA1 / Importin alpha-1 (KPNA1 Produits)

Synonymes: anticorps IPOA5, anticorps NPI-1, anticorps RCH2, anticorps SRP1, anticorps AW494490, anticorps NPI1, anticorps Rch2, anticorps mSRP1, anticorps si:ch211-161h7.7, anticorps karyopherin subunit alpha 1, anticorps importin alpha-1 subunit, anticorps karyopherin (importin) alpha 1, anticorps karyopherin alpha 1 (importin alpha 5), anticorps KPNA1, anticorps Kpna1, anticorps EHI_124900, anticorps kpna1

Sujet: Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta

ID gène: 3836

NCBI Accession: NP_002255

UniProt: P52294

Pathways: M Phase, Protein targeting to Nucleus