KPNA3 anticorps (C-Term)

N° du produit ABIN374393

Détail du produit anti-KPNA3 anticorps

Antigène: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KPNA3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-DPTANLQTKE FNF

Specificité: This antibody detects Importin subunit alpha-3 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_002258.2 Genename: KPNA3

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.5 μg/mL. Approx 55-60 kDa band observed in Human Testis and Kidneylysates (predicted size of approx.50 kDa according to NP_002258). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Heart shows strong pixulate staining ofmyocardial fibres in transverse section.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-KPNA3 (ABIN374393)

Silva, Martins, Tavares, Morgado: "Inhibition of spinal 5-HT3R reverted diabetes-induced mechanical hypersensitivity in a GABAAR-mediated neurotransmission-dependent manner." dans: Neuroscience, Vol. 304, pp. 228-39, (2016) (PubMed).

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." dans: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Ohara, Hata, Yasuhara, Mehmood, Yoneda, Nakagawa, Yamashita: "Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin ?." dans: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 697-702, (2011) (PubMed).

Huenniger, Krämer, Soom, Chang, Köhler, Depping, Kehlenbach, Kaether: "Notch1 signaling is mediated by importins alpha 3, 4, and 7." dans: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 18, pp. 3187-96, (2010) (PubMed).

Détails sur KPNA3

Antigène: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Autre désignation: KPNA3 / Importin alpha-3 (KPNA3 Produits)

Synonymes: anticorps IPOA4, anticorps si:dz79f24.2, anticorps importin, anticorps SRP1, anticorps SRP1gamma, anticorps SRP4, anticorps hSRP1, anticorps ipoa4, anticorps srp1, anticorps srp1gamma, anticorps srp4, anticorps karyopherin (importin) alpha 3, anticorps karyopherin alpha 3 (importin alpha 4), anticorps karyopherin subunit alpha 3, anticorps karyopherin alpha 3 (importin alpha 4) L homeolog, anticorps Kpna3, anticorps kpna3, anticorps KPNA3, anticorps kpna3.L

Sujet: The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that KPNA3 may be involved in the nuclear transport system. The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion, larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.Synonyms: Importin alpha Q2, Karyopherin subunit alpha-3, QIP2, SRP1-gamma

ID gène: 3839

NCBI Accession: NP_002258

UniProt: O00505

Pathways: Protein targeting to Nucleus