TCP1 alpha/CCTA anticorps (C-Term)

N° du produit ABIN374406

Cet anticorps anti-TCP1 alpha/CCTA Polyclonal Chèvre (ABIN374406) détecte spécifiquement TCP1 alpha/CCTA dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TCP1 alpha/CCTA anticorps (C-Term) (ABIN374406)

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TCP1 alpha/CCTA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-TCP1 alpha/CCTA anticorps

Épitope: C-Term

Séquence: C-SYEDAVHSGA LND

Specificité: This antibody detects CCT1 / TCP1 at C-term. It is expected to recognise both reported isoforms.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1. Genename: TCP1

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60 kDa band observed in Hela, Human Lung and HumanTestis lysates (predicted MW of 60 kDa according to NP_110379.2). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows onlycytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining inother germ cells with unstained nucleoli.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur TCP1 alpha/CCTA

Antigène: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Autre désignation: CCT1 / TCP1

Sujet: TCP1 alpha is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.Synonyms: CCT alpha, CCTA, Chaperonin containing T complex polypeptide 1 subunit 1, T-complex protein 1 subunit alpha, TCP-1-alpha, TCP1 alpha

ID gène: 6950

NCBI Accession: NP_110379

UniProt: P17987