NOXO1 anticorps (C-Term)

N° du produit ABIN374411

L’anticorps anti-NOXO1 Polyclonal Chèvre est utilisé pour la détection de NOXO1 dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour NOXO1 anticorps (C-Term) (ABIN374411)

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NOXO1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NOXO1 anticorps

Épitope: C-Term

Séquence: RGCVDSVPHP TTEQ

Specificité: This antibody detects NOXO1 at C-term. It is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1).

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_653204.1, NP_751907.1, NP_751907.1. Genename: NOXO1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 16000. Immunohistochemistry: 10-20 μg/mL. In paraffin embedded Human Kidney Cortex showscytoplasmic stain in epithelial cells of some PCT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Détails sur NOXO1

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Autre désignation: NOXO1

Sujet: NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Cotransfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5 P2, PtdIns 5 P and PtdIns 4 P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 colocalizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes.Synonyms: NADPH oxidase organizer 1, P41NOX, SH3PXD5

ID gène: 124056

NCBI Accession: NP_653204

UniProt: Q8NFA2