PITPNM1 anticorps (C-Term)

N° du produit ABIN374422

Cet anticorps Chèvre Polyclonal détecte spécifiquement PITPNM1 dans WB et IF. Il présente une réactivité avec des échantillons de Humain, Souris, Boeuf (Vache) et Chien.

Aperçu rapide pour PITPNM1 anticorps (C-Term) (ABIN374422)

Antigène: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Reactivité: Humain, Souris, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PITPNM1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-PITPNM1 anticorps

Épitope: C-Term

Séquence: C-KARSISLKLD SEE

Specificité: This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.

Réactivité croisée (Details): Species reactivity (expected):Human, Dog, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C-Terminus of the protein sequence according to NP_004901.2, NP_001124320.1. Genename: PITPNM1

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 8000. Western blot: Customer's experiments gave a band at approx 160 kDa in lysates of a MouseMacrophage cell line after 2 μg/mL antibody staining (calculated MW of 130 kDa accordingto NP_004901.1). In ICC the customer found staining of vesicular cytoplasm in the samecell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PITPNM1

Antigène: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Autre désignation: NIR2

Sujet: NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.Synonyms: DRES9, Membrane-associated phosphatidylinositol transfer protein 1, NIR2, PITPNM, PITPNM1

ID gène: 9600

NCBI Accession: NP_004901

UniProt: O00562