CPEB1 anticorps (C-Term)

N° du produit ABIN374439

Cet anticorps anti-CPEB1 Polyclonal Chèvre (ABIN374439) détecte spécifiquement CPEB1 dans WB. L’anticorps est réactif avec des échantillons de Humain et Macaque.

Aperçu rapide pour CPEB1 anticorps (C-Term) (ABIN374439)

Antigène: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Reactivité: Humain, Macaque

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CPEB1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CPEB1 anticorps

Épitope: C-Term

Séquence: C-PLMRNQKNRD SS

Specificité: This antibody recognizes CPEB1.

Réactivité croisée (Details): Species reactivity (expected):Macaque.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_085097.

Information d'application

Indications d'application: Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL. Approx 65-70 kDa band observed in Human Brain lysates(predicted MW of 60 kDa according to NP_085097). Please note this band was always accompanied by a slightly stonger band at approx 40 kDaand further both bands could be blocked by incubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CPEB1

Antigène: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Autre désignation: CPEB1

Sujet: The regulated translation of messenger RNA is essential for cell cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system. Cytoplasmic polyadenylation, which is one mechanism of controlling translation, is driven by CPEB1 (Cytoplasmic Polyadenylation Element Binding protein), a highly conserved, sequence specific RNA binding protein that binds to the cytoplasmic polyadenylation element (CPE) and modulates translational repression and mRNA localisation. CPEB1 can assemble bothe translational activation and repression complexes via its binding to the CPE. In both vertebrates and invertebrates, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. In Xenopus oocytes, where most of the biochemical details of this process have been examined, polyadenylation is controlled by CPEB1.Synonyms: CPE-BP1, CPE-binding protein 1, Cytoplasmic polyadenylation element-binding protein 1, h-CEBP, hCPEB-1

ID gène: 64506

NCBI Accession: NP_001073001

UniProt: Q9BZB8

Pathways: Synaptic Membrane