RAN anticorps (N-Term)

N° du produit ABIN374447

L’anticorps anti-RAN Polyclonal Chèvre est utilisé pour la détection de RAN dans des échantillons de Humain, Souris, Rat, Chien et Boeuf (Vache). Il a été validé pour WB et EIA.

Aperçu rapide pour RAN anticorps (N-Term) (ABIN374447)

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RAN est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-RAN anticorps

Épitope: N-Term

Séquence: C-AAQGEPQVQF KLV

Specificité: This antibody recognizes Ran (197-210).

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the N-Terminus of the protein sequence according to NP_006316. Genename: RAN

Information d'application

Indications d'application: Peptide ELISA: 1/16,000. Western Blot: 1-3 μg/mL. Detects a band of Approx 25 kDa (Predicted Molecular Weight: 25 kDa). Positive Control: A431 and Jurkat whole cell lysate.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur RAN

Antigène: RAN (RAN, Member RAS Oncogene Family (RAN))

Autre désignation: RAN

Sujet: Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.Synonyms: ARA24, Androgen receptor-associated protein 24, GTP-binding nuclear protein Ran, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein

Poids moléculaire: 24.4 kDa (NP_006316.1)

ID gène: 5901, 9606

UniProt: P62826

Pathways: Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus