FHL1 anticorps (Internal Region)

N° du produit ABIN374471

L’anticorps anti-FHL1 Polyclonal Chèvre est utilisé pour la détection de FHL1 dans des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache) et Porc. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour FHL1 anticorps (Internal Region) (ABIN374471)

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FHL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-FHL1 anticorps

Épitope: Internal Region

Specificité: This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine, Porcine and Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1. Genename: FHL1

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.05-0.3 μg/mL. Detects a band of Approx 30 kDa in Human Muscle lysates(calculated MW of 31.0 kDa according to NP_001440). Immunohistochemistry: 2-2 μg/mL. In paraffin embedded Human Testis shows fibrousstaining in the cytoplasm of developing sperm cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur FHL1

Antigène: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Autre désignation: FHL1

Sujet: FHL1 (SLIM1) is a LIM domain protein transcript that is preferentially expressed in postnatal skeletal muscle but little is known about its function in this tissue. LIM proteins, (named for 'LIN11, ISL1 and MEC3') are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.Synonyms: FHL-1, FHL1, Four and a half LIM domains protein 1, SLIM, SLIM-1, SLIM1, Skeletal Muscle LIM-protein 1

ID gène: 2273

NCBI Accession: NP_001153171

UniProt: Q13642