ADAM12 anticorps (N-Term)

N° du produit ABIN374483

Détail du produit anti-ADAM12 anticorps

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ADAM12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Séquence: AARPLPVSPA RALC

Specificité: This antibody is expected to recognize both the longer membrane-bound form of Human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). It does not cross-react with other ADAMS.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the N Terminus of the protein sequence according to NP_003465, NP_067673

Information d'application

Indications d'application: Peptide ELISA: Detection Limit Dilution: 1/64000. Western blot: 0.1-0.3 μg/mL. Approx 90 kDa band observed in human heart lysates

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ADAM12

Antigène: ADAM12 (ADAM Metallopeptidase Domain 12 (ADAM12))

Autre désignation: ADAM12 / MLTN (ADAM12 Produits)

Synonymes: anticorps ADAM12, anticorps adam12, anticorps Mltna, anticorps mKIAA4001, anticorps MCMP, anticorps MCMPMltna, anticorps MLTN, anticorps MLTNA, anticorps ADAM metallopeptidase domain 12, anticorps a disintegrin and metallopeptidase domain 12 (meltrin alpha), anticorps ADAM12, anticorps adam12, anticorps Adam12

Sujet: ADAM12 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin processed form.Synonyms: Disintegrin and metalloproteinase domain-containing protein 12, Meltrin alpha

ID gène: 8038, 9606

UniProt: O43184

Pathways: EGFR Signaling Pathway