Cet anticorps Chèvre Polyclonal détecte spécifiquement ADH1A dans WB. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Cheval et Chimpanzé.
This antibody is expected to recognize the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of Human alcohol dehydrogenase.
Réactivité croisée (Details)
Species reactivity (expected):Mouse, Rat. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogène
Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
ADH1A
Reactivité: Humain
Hôte: Lapin
Polyclonal
FITC
Indications d'application
Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 μg/mL. Approx 38 kDa band observed in Human Liver lysates(calculated MW of 39.8 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as presrevative and 0.5 % BSA as stabilizer.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains of three types, alpha, beta and gamma. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.Synonyms: ADH1A, Alcohol dehydrogenase subunit alpha