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ADH1A anticorps (N-Term)

Cet anticorps Chèvre Polyclonal détecte spécifiquement ADH1A dans WB. Il présente une réactivité envers Humain, Souris, Rat, Cheval et Chimpanzé.
N° du produit ABIN374506

Aperçu rapide pour ADH1A anticorps (N-Term) (ABIN374506)

Antigène

Voir toutes ADH1A Anticorps
ADH1A (Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A))

Reactivité

  • 26
  • 13
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Humain, Souris, Rat, Cheval, Chimpanzé

Hôte

  • 25
  • 2
Chèvre

Clonalité

  • 24
  • 3
Polyclonal

Conjugué

  • 16
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp ADH1A est non-conjugé

Application

  • 15
  • 9
  • 6
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Épitope

    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    Specificité

    This antibody is expected to recognize the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of Human alcohol dehydrogenase.

    Réactivité croisée (Details)

    Species reactivity (expected):Mouse, Rat.
    Species reactivity (tested):Human.

    Purification

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    Immunogène

    Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
  • Indications d'application

    Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 μg/mL. Approx 38 kDa band observed in Human Liver lysates(calculated MW of 39.8 kDa according to NP_000658.1, NP_000659.2 and 000660.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    0.5 mg/mL

    Buffer

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as presrevative and 0.5 % BSA as stabilizer.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Antigène

    ADH1A (Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A))

    Autre désignation

    Alcohol Dehydrogenase 1A (ADH1)

    Sujet

    ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains of three types, alpha, beta and gamma. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.Synonyms: ADH1A, Alcohol dehydrogenase subunit alpha

    ID gène

    124, 9606

    UniProt

    P07327
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