POMC anticorps (C-Term)

N° du produit ABIN374549

L’anticorps anti-POMC Polyclonal Chèvre est utilisé pour la détection de POMC dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour POMC anticorps (C-Term) (ABIN374549)

Antigène: POMC (Proopiomelanocortin (POMC))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POMC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-POMC anticorps

Épitope: C-Term

Séquence: C-NAIIKNAYKK GE

Specificité: In Western blot, this antibody appears to react with the full length precursor protein only.

Réactivité croisée (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human and Mouse.

Purification: Immunoaffinity Chromatography

Immunogène: Synthetic Peptide corresponding to C-Terminal of Human POMC (according to NP_000930.1, NP_001030333.1) Genename: POMC

Information d'application

Indications d'application: Peptide ELISA: Detection Limit Dilution: 1/32,000. Western Blot: Use at 0.01-0.03 μg/mL. Detects a band of approx. 26 kDa band in lysates of Mouse pituitary cell line NIH3T3. Immunohistochemistry: Use at 2-4 μg/mL. In Paraffin Embedded Human Adrenal Gland shows strong cytoplasm staining in selectcells in the medulla.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.

Détails sur POMC

Antigène: POMC (Proopiomelanocortin (POMC))

Autre désignation: POMC

Sujet: POMC is a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used, adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. Mutations in POMC gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.Synonyms: Corticotropin-Lipotropin, Pro-opiomelanocortin

Poids moléculaire: 29.4kDa (Human NP_000930.1) and 26.7kDa (Mouse NP_032921.1).

ID gène: 5443, 9606

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour