PPY anticorps (Internal Region)

N° du produit ABIN374577

Cet anticorps anti-PPY Polyclonal Chèvre (ABIN374577) détecte spécifiquement PPY dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat, Souris et Chien.

Aperçu rapide pour PPY anticorps (Internal Region) (ABIN374577)

Antigène: PPY (Pancreatic Polypeptide (PPY))

Reactivité: Humain, Rat, Souris, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PPY est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PPY anticorps

Épitope: Internal Region

Séquence: C-TRPRYGKRHK EDT

Specificité: This antibody recognizes Pancreatic prohormone.

Réactivité croisée (Details): Species reactivity (expected):Rat and Canine (Dog).
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Synthetic peptide from the internal region of the protein sequence according to NP_002713.1. Genename: PPY

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Western Blot: Preliminary experiments gave an approx 35-38 kDa band in human pancreaslysates after 1 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 10.8 kDaaccording to NP_002713.1. The 38 kDa band was successfully blocked by incubation withthe immunizing peptide. Immunohistochemistry: 3 μg/mL. In paraffin embedded human pancreas, shows strong cytoplasmic staining of rare cellslocated at the periphery of islets of Langerhans.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PPY

Antigène: PPY (Pancreatic Polypeptide (PPY))

Autre désignation: Pancreatic Prohormone

Sujet: Pancreatic hormone (PPY) is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma levels of this hormone have been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.Synonyms: Obinepitide, PNP, PPY, Pancreatic polypeptide

ID gène: 5539

NCBI Accession: NP_002713

UniProt: P01298

Pathways: Feeding Behaviour