DAZL anticorps (Internal Region)

N° du produit ABIN374590

Cet anticorps Chèvre Polyclonal détecte spécifiquement DAZL dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour DAZL anticorps (Internal Region) (ABIN374590)

Antigène: DAZL (Deleted in Azoospermia-Like (DAZL))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DAZL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DAZL anticorps

Épitope: Internal Region

Séquence: C-GNGPQKKSVD R

Specificité: This antibody recognizes DAZL.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Synthetic peptide corresponding to the internal sequence of Human DAZL according to NP_001342.2 Genename: DAZL

Information d'application

Indications d'application: Peptide ELISA: 1/64000. Western blot: Approx 35 kDa band observed in human ovary and human testes lysates(calculated MW of 33.5 kDa according to NP_001342.2). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Mouse Testis shows cytoplasm staining ofspermatogonia. Additional staining is observed in the cytoplasm of elongatng spermatidsbut this additional staining is not in agreement with results obtained by another antibody(Ruggiuet al, Nature.1997 Sep 4,389(6646): 73-7. PMID: 9288969). Data kindly provided by J. S. Martins, MRC, Edinburgh, UK. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur DAZL

Antigène: DAZL (Deleted in Azoospermia-Like (DAZL))

Autre désignation: DAZL

Sujet: DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males.Synonyms: DAZ homolog, DAZ-like autosomal, DAZH, DAZL, DAZL1, DAZLA, Deleted in azoospermia-like, Deleted in azoospermia-like 1, SPGY-like-autosomal, SPGYLA

ID gène: 1618

NCBI Accession: NP_001342

UniProt: Q92904