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PEPCK anticorps (Internal Region)

PEPCK Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Chien WB, EIA Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN374610
  • Antigène Voir toutes PEPCK Anticorps
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Épitope
    • 15
    • 9
    • 8
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    Humain, Souris, Rat, Porc, Boeuf (Vache), Chien
    Hôte
    • 60
    • 7
    • 1
    Chèvre
    Clonalité
    • 54
    • 14
    Polyclonal
    Conjugué
    • 27
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PEPCK est non-conjugé
    Application
    • 51
    • 27
    • 19
    • 14
    • 14
    • 10
    • 7
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Séquence
    KPWKPGDKEP CAH
    Specificité
    This antibody recognizes PCK2. This antibody is expected to recognise isoform 1 only, no crossreactivity with PCK1.
    Réactivité croisée (Details)
    Species reactivity (expected):Canine, Bovine, Porcine.
    Species reactivity (tested):Human, Mouse, Rat, Guinea Pig.
    Purification
    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
    Immunogène
    Peptide with sequence from the internal region of the protein sequence according to NP_004554.2. Genename: PCK2
    Top Product
    Discover our top product PEPCK Anticorps primaire
  • Indications d'application
    Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Liver lysates and inlysates of cell line HepG2 (calculated MW of 70.0 kDa according to NP_004554.2).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." dans: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

    Idnurm: "Light sensing in Aspergillus fumigatus highlights the case for establishing new models for fungal photobiology." dans: mBio, Vol. 4, Issue 3, pp. e00260-13, (2013) (PubMed).

  • Antigène
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Autre désignation
    PCK2 (PEPCK Produits)
    Synonymes
    anticorps PEPCK, anticorps PEPCK-M, anticorps PEPCK2, anticorps 1810010O14Rik, anticorps 9130022B02Rik, anticorps pck, anticorps zgc:77867, anticorps id:ibd5157, anticorps pck2, anticorps pck2.S, anticorps phosphoenolpyruvate carboxykinase 2, mitochondrial, anticorps phosphoenolpyruvate carboxykinase 2 (mitochondrial), anticorps phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog, anticorps PCK2, anticorps Pck2, anticorps pck2, anticorps pck2.L
    Sujet
    PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase
    ID gène
    5106
    NCBI Accession
    NP_001018083
    UniProt
    Q16822
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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