PEPCK anticorps (Internal Region)

N° du produit ABIN374610

Cité dans 2 publications. Cet anticorps anti-PEPCK Polyclonal Chèvre (ABIN374610) détecte spécifiquement PEPCK dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour PEPCK anticorps (Internal Region) (ABIN374610)

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PEPCK est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-PEPCK anticorps

Épitope: Internal Region

Séquence: KPWKPGDKEP CAH

Specificité: This antibody recognizes PCK2. This antibody is expected to recognise isoform 1 only, no crossreactivity with PCK1.

Réactivité croisée (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse, Rat, Guinea Pig.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_004554.2. Genename: PCK2

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Liver lysates and inlysates of cell line HepG2 (calculated MW of 70.0 kDa according to NP_004554.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-PEPCK (ABIN374610)

Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." dans: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

Idnurm: "Light sensing in Aspergillus fumigatus highlights the case for establishing new models for fungal photobiology." dans: mBio, Vol. 4, Issue 3, pp. e00260-13, (2013) (PubMed).

Détails sur PEPCK

Antigène: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Autre désignation: PCK2

Sujet: PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase

ID gène: 5106

NCBI Accession: NP_001018083

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis