PRNP anticorps (Internal Region)

N° du produit ABIN374705

L’anticorps anti-PRNP Polyclonal Chèvre est utilisé pour la détection de PRNP dans des échantillons de Humain, Boeuf (Vache) et Porc. Il a été validé pour WB et EIA.

Aperçu rapide pour PRNP anticorps (Internal Region) (ABIN374705)

Antigène: PRNP (Prion Protein (PRNP))

Reactivité: Humain, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PRNP est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-PRNP anticorps

Épitope: AA 143-153, Internal Region

Séquence: C-SDYEDRYYRE N

Specificité: This antibody detects an internal region of Prion Protein.

Réactivité croisée (Details): Species reactivity (expected):Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_000302.1. Genename: PRNP

Information d'application

Indications d'application: ELISA: 1/64000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PRNP

Antigène: PRNP (Prion Protein (PRNP))

Autre désignation: CD230 / PrP

Sujet: Prion protein PrP is a membrane glycosylphosphatidylinositol anchored glycoprotein that tends to aggregate into rod like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt Jakob disease, fatal familial insomnia, Gerstmann Straussler disease, Huntington disease like 1, and kuru. Two transcript variants encoding the same protein have been found for this gene. All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPsc). Both PrPsc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids. Many mammalian PrPs have a 22 amino acid N terminal signal sequence and 23 amino acid C terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor. The mature protein of 209 amino acids contains one disulfide bond and has two sites of asparagine linked glycosylation.Synonyms: ASCR, Major prion protein, PRIP, PRNP, PrP27-30, PrP33-35C

ID gène: 5621, 9606

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation