GPX4 anticorps (C-Term, Isoform A)

N° du produit ABIN374711

L’anticorps anti-GPX4 Polyclonal Chèvre est utilisé pour la détection de GPX4 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Porc. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour GPX4 anticorps (C-Term, Isoform A) (ABIN374711)

Antigène: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GPX4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-GPX4 anticorps

Épitope: C-Term, Isoform A

Specificité: This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Pig, Cow.
Species reactivity (tested):Human.

Purification: Affinity Chromatpography

Immunogène: Peptide with sequence (C-EEPLVIEKDLPHY) from the C-terminus of the protein sequencen (NP_002076.2, NP_001034937.1).

Information d'application

Indications d'application: ELISA: 1/16000. Western Blot: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur GPX4

Antigène: GPX4 (Glutathione Peroxidase 4 (GPX4))

Autre désignation: Glutathione Peroxidase 4 / GPX4

Sujet: Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial

ID gène: 2879

NCBI Accession: NP_001034936

UniProt: P36969