CCKAR anticorps (Internal Region)

N° du produit ABIN374792

L’anticorps anti-CCKAR Polyclonal Chèvre est utilisé pour la détection de CCKAR dans des échantillons de Rat, Souris et Lapin. Il a été validé pour WB.

Aperçu rapide pour CCKAR anticorps (Internal Region) (ABIN374792)

Antigène: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reactivité: Rat, Souris, Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CCKAR est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CCKAR anticorps

Épitope: Internal Region

Séquence: C-KFDASQKKSA KEKR

Specificité: This antibody detects CCK-A Receptor.

Réactivité croisée (Details): Species reactivity (expected):Mouse, RabbitSpecies reactivity (tested):Rat.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the internal region of the protein sequence according to NP_033957.1. Genename: Cckar

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Approx.75 kDa band observed in purified membranes of Ratinsulinoma cell line RIN14B (calculated MW of 48.5 kDa according to NP_033957.1). Datakindly provided by Dr. Morisset, Sherbrooke, Canada. Data accepted by InternationalJournal of Endocrinology (in press). The observed molecular weight also corresponds toearlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem.2003 Nov,51(11): 1501-13, PMID: 14566022).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide, with 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CCKAR

Antigène: CCKAR (Cholecystokinin A Receptor (CCKAR))

Autre désignation: CCK-A Receptor

Sujet: The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous important physiologic events. These appear to be mediated through 2 general classes of receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family peptides. Through binding to class A receptors, CCK is a major physiologic mediator of gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion. Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the central nervous system, where they have been implicated in the pathogenesis of feeding disorders, Parkinson disease, schizophrenia, and drug addiction.Synonyms: CCKA Receptor, CCKAR, CCKRA, Cholecystokinin receptor type A, Cholecystokinin-1 receptor

ID gène: 886, 9606

UniProt: P32238

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour