Nanog anticorps (C-Term)

N° du produit ABIN374808

Cet anticorps anti-Nanog Polyclonal Chèvre (ABIN374808) détecte spécifiquement Nanog dans WB, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Nanog anticorps (C-Term) (ABIN374808)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Nanog anticorps

Épitope: C-Term

Séquence: C-DLFLNYSMNM QPED

Specificité: Recognizes Human NANOG (C-term). Others species not tesetd.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the C Terminus of the protein sequence according to NP_079141.1.

Information d'application

Indications d'application: Peptide ELISA: 1/128000. Western blot: 0.05-0.1 μg/mL. Approx 35 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 34.5 kDa according to NP_079141.1). An additional band of approx.35 kDa is observed and is consistent with theobservations of higher molecular weight bands regularly found with antibodies from othersources. Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Testis shows nuclear staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: NANOG

Sujet: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

ID gène: 79923, 9606

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance