ADH5 anticorps (Internal Region)

N° du produit ABIN374869

Cet anticorps Chèvre Polyclonal détecte spécifiquement ADH5 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Porc, Boeuf (Vache) et Chien.

Aperçu rapide pour ADH5 anticorps (Internal Region) (ABIN374869)

Antigène: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ADH5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-ADH5 anticorps

Épitope: Internal Region

Séquence: C-KKIKVDEFVT HN

Specificité: This antibody detects Alcohol Dehydrogenase 5 (ADH5).

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5

Information d'application

Indications d'application: Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ADH5

Antigène: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Autre désignation: Alcohol Dehydrogenase 5 (ADH5)

Sujet: The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Poids moléculaire: 39.7 kDa according to NP_000662.3.

ID gène: 128, 9606

UniProt: P11766