FTH1 anticorps (C-Term, Heavy Chain)

N° du produit ABIN375067

Cet anticorps Chèvre Polyclonal détecte spécifiquement FTH1 dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain et Chien.Il a été cité dans 1 publication.

Aperçu rapide pour FTH1 anticorps (C-Term, Heavy Chain) (ABIN375067)

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivité: Humain, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FTH1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-FTH1 anticorps

Épitope: C-Term, Heavy Chain

Séquence: C-DKHTLGDSDN ES

Specificité: This antibody recognizes FTH1 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the C-Terminus of the protein sequence according to NP_002023.2. Genename: FTH1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.03-0.1 μg/mL. Approx.22 kDa band observed in Human Brain (Cerebellum),Liver and Placenta lysates. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-FTH1 (ABIN375067)

Sammarco, Ditch, Banerjee, Grabczyk: "Ferritin L and H subunits are differentially regulated on a post-transcriptional level." dans: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4578-87, (2008) (PubMed).

Détails sur FTH1

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Autre désignation: Ferritin Heavy Chain (FTH1)

Sujet: Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy, 21 kDa) and L (light, 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.Synonyms: Cell proliferation-inducing gene 15 protein, EC=1.16.3.1, FTH, FTH-1, FTHL6, Ferritin H subunit

Poids moléculaire: 21.2kDa (NP_002023.2)

ID gène: 2495

NCBI Accession: NP_002023

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis