EGR2 anticorps (Internal Region)

N° du produit ABIN375194

L’anticorps anti-EGR2 Polyclonal Chèvre est utilisé pour la détection de EGR2 dans des échantillons de Humain, Souris, Rat et Chien. Il a été validé pour WB, IHC (p) et EIA.

Aperçu rapide pour EGR2 anticorps (Internal Region) (ABIN375194)

Antigène: EGR2 (Early Growth Response 2 (EGR2))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EGR2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-EGR2 anticorps

Épitope: Internal Region

Séquence: HGTAGPDRKP FPC

Specificité: This antibody recognizes EGR2

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_000390.2. Genename: EGR2

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EGR2

Antigène: EGR2 (Early Growth Response 2 (EGR2))

Autre désignation: EGR2

Sujet: ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20

ID gène: 1959

NCBI Accession: NP_000390

UniProt: P11161