DYKDDDDK Tag anticorps

N° du produit ABIN387700

Cet anticorps Souris Monoclonal détecte spécifiquement DYKDDDDK Tag dans WB, ELISA et IP. Il a été cité dans 2+ publications.

Aperçu rapide pour DYKDDDDK Tag anticorps (ABIN387700)

Antigène: DYKDDDDK Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYKDDDDK Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 5A8E5

Détail du produit anti-DYKDDDDK Tag anticorps

Séquence: DYKDDDDK

Specificité: THE^TM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.

Attributs du produit: Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.

Purification: Protein A affinity column

Immunogène: A synthetic peptide (DYKDDDDK) coupled - KLH

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.

Références pour anticorps anti-DYKDDDDK Tag (ABIN387700)

Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." dans: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).

Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." dans: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Classe de substances: Tag

Sujet: The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.