His Tag anticorps (HRP)

N° du produit ABIN387701

Détail du produit anti-His Tag anticorps (HRP)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Marque: THE™

Séquence: HHHHHH

Specificité: THE^TM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Attributs du produit: This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.

Purification: Protein A affinity column

Immunogène: A synthetic peptide HHHHHH coupled - KLH

Clone: 6G2A9

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-His Tag (HRP) (ABIN387701)

Samelson, Gore, Whiting, Nygren, Purkey, Colledge, Langeberg, DellAcqua, Zweifel, Scott: "A-kinase Anchoring Protein 79/150 Recruits Protein Kinase C to Phosphorylate Roundabout Receptors." dans: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14107-19, (2015) (PubMed).

Almeida, Abreu, Matos, Fraga, Fernandes, Macedo, Gutiérrez-Gallego, Pereira, Carvalho, Macedo-Ribeiro: "SUMOylation of the brain-predominant Ataxin-3 isoform modulates its interaction with p97." dans: Biochimica et biophysica acta, Vol. 1852, Issue 9, pp. 1950-9, (2015) (PubMed).

Isik, Sliepen, van Montfort, Sanders: "Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL." dans: PLoS ONE, Vol. 9, Issue 9, pp. e107683, (2014) (PubMed).

Vire, Skarzynski, Thomas, Nelson, David, Aue, Burke, Rader, Wiestner: "Harnessing the fc? receptor for potent and selective cytotoxic therapy of chronic lymphocytic leukemia." dans: Cancer research, Vol. 74, Issue 24, pp. 7510-20, (2014) (PubMed).

Melnikow, Xu, Liu, Bell, Ghedin, Unnasch, Lustigman: "A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis." dans: PLoS neglected tropical diseases, Vol. 7, Issue 4, pp. e2151, (2013) (PubMed).

Jarmander, Gustavsson, Do, Samuelson, Larsson: "A dual tag system for facilitated detection of surface expressed proteins in Escherichia coli." dans: Microbial cell factories, Vol. 11, pp. 118, (2012) (PubMed).

Détails sur His Tag

Antigène: His Tag

Abstract: His Tag Produits

Classe de substances: Tag