His Tag anticorps (Biotin)

N° du produit ABIN387702

Détail du produit anti-His Tag anticorps (Biotin)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Marque: THE™

Séquence: HHHHHH

Specificité: THE^TM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Attributs du produit: The antibody is suitable for detecting fusion proteins that contain a His-tag and it is supplied as a 100 µL aliquot at a concentration of 1 mg/mL.

Purification: Protein A affinity column

Immunogène: A synthetic peptide (HHHHHH) coupled - KLH

Clone: 6G2A9

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Références pour anticorps anti-His Tag (Biotin) (ABIN387702)

Wegman, Geuze, van der Helm, Cumhur Öner, Dhert, Alblas: "Gene delivery of bone morphogenetic protein-2 plasmid DNA promotes bone formation in a large animal model." dans: Journal of tissue engineering and regenerative medicine, Vol. 8, Issue 10, pp. 763-70, (2014) (PubMed).

Ota, Doyle-Cooper, Cooper, Huber, Falkowska, Doores, Hangartner, Le, Sok, Jardine, Lifson, Wu, Mascola, Poignard, Binley, Chakrabarti, Schief, Wyatt, Burton, Nemazee: "Anti-HIV B Cell lines as candidate vaccine biosensors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 10, pp. 4816-24, (2012) (PubMed).

Détails sur His Tag

Antigène: His Tag

Abstract: His Tag Produits

Classe de substances: Tag