Myc Tag anticorps

Détails pour le produit réf. ABIN387746, Fournisseur: Connectez-vous pour afficher
Antigène
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • ARC
  • B430311C09Rik
  • MYC
  • NOP
  • Nop30
  • MYC proto-oncogene, bHLH transcription factor
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • MYC
  • Nol3
Reactivité
Tag
228
62
17
12
6
4
3
3
1
Hôte
Souris
183
85
19
16
8
6
3
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
30
24
14
8
5
5
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
278
125
117
77
55
39
35
34
25
11
7
6
6
6
6
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Options
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N° du produit (Fournisseur)
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Immunogène KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Séquence EQKLISEEDL
Clone 9E10
Isotype IgG1
Specificité KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Antigène
Classe de substances Tag
Sujet Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells.
Synonyms: Tag from c-Myc protein
Poids moléculaire 48 kDA
Indications d'application WB = 1:2, 000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption 6 months
Images (Fournisseur)
 image for anti-Myc Tag antibody (ABIN387746) Myc-tagged recombinant protein was expressed in COS7 cells. 20 ug of cell lysate was ...
Western Blotting (WB) image for anti-Myc Tag antibody (ABIN387746) Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(ABIN387746,ABI...
Western Blotting (WB) image for anti-Myc Tag antibody (ABIN387746) Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/...
Produit citée dans: Brocard, Dufour, Gory-Fauré, Arnoult, Bosc, Denarier, Peris, Saoudi, De Waard, Andrieux: "MAP6 interacts with Tctex1 and Cav 2.2/N-type calcium channels to regulate calcium signalling in neurons." dans: The European journal of neuroscience, Vol. 46, Issue 11, pp. 2754-2767, 2018 (PubMed). Méthode utilisée: Western Blotting (WB)

Zheng, Wang, Weighill, Guo, Xie, Yang, Yang, Wang, Jacobson, Guo, Muchero, Tuskan, Chen: "Characterization of DWARF14 Genes in Populus." dans: Scientific reports, Vol. 6, pp. 21593, 2017 (PubMed). Méthode utilisée: Western Blotting (WB)

Xu, Jin, Hu, Wang, Zhang, Yuan, Cao: "The Joubert Syndrome Protein Inpp5e Controls Ciliogenesis by Regulating Phosphoinositides at the Apical Membrane." dans: Journal of the American Society of Nephrology : JASN, Vol. 28, Issue 1, pp. 118-129, 2017 (PubMed). Méthode utilisée: Western Blotting (WB)

Chang, Hsu, Weng, Chen, Wang, Chou, Liu, Yuan, Huang, Lin, Chen, Tsai: "N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability." dans: International journal of molecular sciences, Vol. 17, Issue 6, 2016 (PubMed). Méthode utilisée: Western Blotting (WB)

Wang, Zhang, Dou, Zhang, Chen, Yu, Ren: "Synergistic and independent actions of multiple terminal nucleotidyl transferases in the 3' tailing of small RNAs in Arabidopsis." dans: PLoS genetics, Vol. 11, Issue 4, pp. e1005091, 2015 (PubMed). Méthode utilisée: Western Blotting (WB)

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." dans: Journal of integrative plant biology, 2015 (PubMed). Méthode utilisée: Chromatin Immunoprecipitation (ChIP)

Hu, Yu, Zhou, Li, Xia, Luo, Wu: "A small GTPase?like protein fragment of Mycoplasma promotes tumor cell migration and proliferation in vitro via interaction with Rac1 and Stat3." dans: Molecular medicine reports, Vol. 9, Issue 1, pp. 173-9, 2013 (PubMed). Méthode utilisée: Western Blotting (WB)

Liu, Yan, Liu, Hong, Sun, Shi, Huang, Fang: "Complementation of HYPONASTIC LEAVES1 by double-strand RNA-binding domains of DICER-LIKE1 in nuclear dicing bodies." dans: Plant physiology, Vol. 163, Issue 1, pp. 108-17, 2013 (PubMed). Méthode utilisée: Western Blotting (WB)

Chu, Loughlin, Gaur, SenBanerjee, Jacob, Monson, Kent, Oranu, Ding, Ukomadu, Sadler: "UHRF1 phosphorylation by cyclin A2/cyclin-dependent kinase 2 is required for zebrafish embryogenesis." dans: Molecular biology of the cell, Vol. 23, Issue 1, pp. 59-70, 2012 (PubMed). Méthode utilisée: Western Blotting (WB)

Chu, Mir, Gao, Rosa, Monson, Sharma, Steet, Freeze, Lehrman, Sadler: "A zebrafish model of congenital disorders of glycosylation with phosphomannose isomerase deficiency reveals an early opportunity for corrective mannose supplementation." dans: Disease models & mechanisms, Vol. 6, Issue 1, pp. 95-105, 2012 (PubMed). Méthode utilisée: Western Blotting (WB)

Hakozaki, Yoda, Tohmonda, Furukawa, Hikata, Uchikawa, Takaishi, Matsumoto, Chiba, Horiuchi, Toyama: "Receptor activator of NF-kappaB (RANK) ligand induces ectodomain shedding of RANK in murine RAW264.7 macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 5, pp. 2442-8, 2010 (PubMed). Méthode utilisée: Western Blotting (WB)

Satow, Shitashige, Jigami, Honda, Ono, Hirohashi, Yamada: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." dans: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, 2010 (PubMed). Méthode utilisée: Western Blotting (WB)

Kim, Kang, Nagaraj, Blancaflor, Mysore, Chapman: "Mutations in Arabidopsis fatty acid amide hydrolase reveal that catalytic activity influences growth but not sensitivity to abscisic acid or pathogens." dans: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34065-74, 2009 (PubMed). Méthode utilisée: Western Blotting (WB)

Kobayashi, Barnard, Said, Hong-Gonzalez, Corman, Ku, Doan, Gui, Elashoff, Cohen, Aronson: "Effect of low-fat diet on development of prostate cancer and Akt phosphorylation in the Hi-Myc transgenic mouse model." dans: Cancer research, Vol. 68, Issue 8, pp. 3066-73, 2008 (PubMed).

Zemskov, Mikhailenko, Strickland, Belkin: "Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1." dans: Journal of cell science, Vol. 120, Issue Pt 18, pp. 3188-99, 2007 (PubMed). Méthode utilisée: Western Blotting (WB)

Hang, Zemskov, Lorand, Belkin: "Identification of a novel recognition sequence for fibronectin within the NH2-terminal beta-sandwich domain of tissue transglutaminase." dans: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23675-83, 2005 (PubMed). Méthode utilisée: Western Blotting (WB)

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