HA-Tag anticorps

N° du produit ABIN387747

Cet anticorps anti-HA-Tag est un anticorps Souris Monoclonal détectant HA-Tag dans WB. Adapté pour Humain. Ce Primary Antibody a été cité dans 24+ publications.

Aperçu rapide pour HA-Tag anticorps (ABIN387747)

Antigène: HA-Tag

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HA-Tag est non-conjugé

Application: Western Blotting (WB)

Clone: 12CA5

Détail du produit anti-HA-Tag anticorps

Séquence: YPYDVPDYA

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogène: KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1:8000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Date de péremption: 6 months

Références pour anticorps anti-HA-Tag (ABIN387747)

Milewska, Kindler, Vkovski, Zeglen, Ochman, Thiel, Rajfur, Pyrc: "APOBEC3-mediated restriction of RNA virus replication." dans: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).

Li, Guo, Feng, Zhang, Wang, Jing, Yan, Jing, Liu, Ma, Diao: "Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation." dans: Cell proliferation, Vol. 49, Issue 6, pp. 710-719, (2017) (PubMed).

Tham, Joshi, Moukhles: "Aquaporin-4 Cell-Surface Expression and Turnover Are Regulated by Dystroglycan, Dynamin, and the Extracellular Matrix in Astrocytes." dans: PLoS ONE, Vol. 11, Issue 10, pp. e0165439, (2017) (PubMed).

Schmidt, Goebeler, Martin: "Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis." dans: Methods in molecular biology (Clifton, N.J.), Vol. 1390, pp. 319-40, (2016) (PubMed).

Mertz, Tan, Pagala, Bai, Chen, Li, Cho, Shaw, Wang, Peng: "Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth." dans: Molecular & cellular proteomics : MCP, Vol. 14, Issue 7, pp. 1898-910, (2015) (PubMed).

Mizutani, Inoue, Omori, Ito, Tamiya-Koizumi, Takagi, Kojima, Nakamura, Iwaki, Nakatochi, Suzuki, Nozawa, Murate: "Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP." dans: Journal of biochemistry, (2015) (PubMed).

Drechsler, Tan, Liakopoulos: "Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2." dans: Journal of cell science, Vol. 128, Issue 21, pp. 3910-21, (2015) (PubMed).

Li, An, Li, Ma, Feng, Zhang, Guo: "DELLA Proteins Interact with FLC to Repress the Flowering Transition." dans: Journal of integrative plant biology, (2015) (PubMed).

Koo, Yue, Gal, Khuri, Sun: "Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth." dans: Cancer research, Vol. 74, Issue 9, pp. 2555-68, (2014) (PubMed).

Bruntz, Taylor, Lindsley, Brown: "Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells." dans: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 600-16, (2014) (PubMed).

Li, Ren, Yue, Chen, Khuri, Sun: "The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition." dans: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 4, pp. 612-20, (2012) (PubMed).

Sun, Ni: "HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation." dans: Molecular plant, Vol. 4, Issue 1, pp. 116-26, (2011) (PubMed).

Czymai, Viemann, Sticht, Molema, Goebeler, Schmidt: "FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms." dans: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10163-78, (2010) (PubMed).

Herrera, Silva, Garvin: "Angiotensin II stimulates thick ascending limb superoxide production via protein kinase C(?)-dependent NADPH oxidase activation." dans: The Journal of biological chemistry, Vol. 285, Issue 28, pp. 21323-8, (2010) (PubMed).

Kauh, Fan, Xia, Yue, Yang, Khuri, Sun: "c-FLIP degradation mediates sensitization of pancreatic cancer cells to TRAIL-induced apoptosis by the histone deacetylase inhibitor LBH589." dans: PLoS ONE, Vol. 5, Issue 4, pp. e10376, (2010) (PubMed).

Nishikawa, Nakashima, Hayashi, Fukunaga, Kato, Kodama, Takahashi, Calame, Takayanagi: "Blimp1-mediated repression of negative regulators is required for osteoclast differentiation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 7, pp. 3117-22, (2010) (PubMed).

Flozak, Lam, Russell, Jain, Peled, Sheppard, Beri, Mutlu, Budinger, Gottardi: "Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells." dans: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3157-67, (2010) (PubMed).

Satow, Shitashige, Jigami, Honda, Ono, Hirohashi, Yamada: "Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation." dans: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26289-94, (2010) (PubMed).

Guo, Thomas, Collins, Timmermans: "Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis." dans: The Plant cell, Vol. 20, Issue 1, pp. 48-58, (2008) (PubMed).

Chen, Liu, Yue, Schönthal, Khuri, Sun et al.: "CCAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction and ubiquitin/proteasome-mediated cellular FLICE-inhibitory protein down-regulation contribute to enhancement of ..." dans: Molecular pharmacology, Vol. 72, Issue 5, pp. 1269-79, (2007) (PubMed).

Détails sur HA-Tag

Antigène: HA-Tag

Classe de substances: Tag

Sujet: Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.