ATG7 anticorps (AA 284-313)

N° du produit ABIN388521

Cité dans 2 publications. Cet anticorps anti-ATG7 Polyclonal Lapin (Clone RB7469) (ABIN388521) détecte spécifiquement ATG7 dans WB et IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour ATG7 anticorps (AA 284-313) (ABIN388521)

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB7469

Détail du produit anti-ATG7 anticorps

Épitope: AA 284-313

Homologie: C, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 284-313 amino acids from the Central region of human ATG7.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-ATG7 (ABIN388521)

Ma, Molusky, Song, Hu, Fang, Rui, Mathew, Pennathur, Liu, Cheng, Guan, Lin: "Autophagy deficiency by hepatic FIP200 deletion uncouples steatosis from liver injury in NAFLD." dans: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 10, pp. 1643-54, (2013) (PubMed).

Ma, Panda, Lin: "Temporal orchestration of circadian autophagy rhythm by C/EBP?." dans: The EMBO journal, Vol. 30, Issue 22, pp. 4642-51, (2012) (PubMed).

Détails sur ATG7

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: ATG7

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Poids moléculaire: 77960

ID gène: 10533

NCBI Accession: NP_001129503, NP_001138384, NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagy